Figure 3. Lack of IL27 signaling stabilizes mutant p53 in vivo and in vitro.

(A) p53 staining via immunohistochemistry of bone marrow vertebral bodies from 8 month old wildtype, IL27RA^−/− p53^R172H/+ , and IL27RA^−/− p53^H/+ mice. (B) p53 and p21 staining via immunohistochemistry of bone marrow vertebral bodies from 2, 4, and 8 month old IL27RA^−/− p53^H/+ mice including a positive control for p21. (C) Bone marrow cells derived from aged wildtype, p53^H/+ and IL27RA^−/−p53^H/+ mice were seeded in a 6-well plate and either irradiated (IR) or left un-irradiated. Cells were collected at the indicated time points and were probed for p53 and actin. (D) H318 and H76 osteosarcoma cells were treated with rIL27 for 30 mins at 20ng/ml and collected lysates were probed for p53 levels and actin,