Figure 4. Absence of IL27 signaling increases macrophage and neo-angiogenesis marker Endoglin in mutant p53-driven osteosarcomas.

(A) No significant changes were seen in mice of different genotypes in CD4, CD8, B, NK, myeloid, CD11c cells in the bone marrow of 3 month old mice bearing indicated genetic modifications as analyzed by flow cytometry. Significant changes were seen in the number of macrophages in the bone marrow (B) present in the aged IL27RA^−/−p53^H/+ mice when compared to age-matched control ones analyzed via immunohistochemistry. (C-D) No differences in the proliferation rate of these end-stage tumors were noted (C, Ki67 staining), but a higher number of endoglin-positive cells in osteosarcomas from IL27RA^−/−p53^H/+. Representative migrographs from at least 3 independent mice. Dots indicate data from the bone marrow of individual mice. Pictures takes at 400X. *, p<0.05