Skip to main content
. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Clin Cancer Res. 2016 Mar 8;22(15):3841–3848. doi: 10.1158/1078-0432.CCR-15-2173

Table 3.

Tumor MIR21 expression level and colorectal cancer mortality according to PTGS2 expression

No. of cases Colorectal cancer-specific mortality
Overall mortality
No. of events Univariable
HR (95% CI)
Multivariable
HR (95% CI)a
No. of events Univariable
HR (95% CI)
Multivariable
HR (95% CI)a
PTGS2-absent/low cancer
MIR21 expression level
 Quartile 1 (lowest) 73 25 1 (reference) 1 (reference) 44 1 (reference) 1 (reference)
 Quartile 2 73 14 0.49 (0.26–0.95) 0.57 (0.29–1.11) 33 0.63 (0.40–1.00) 0.71 (0.45–1.12)
 Quartile 3 76 26 0.99 (0.57–1.71) 0.90 (0.52–1.57) 43 1.07 (0.70–1.63) 1.01 (0.66–1.56)
 Quartile 4 (highest) 73 22 0.91 (0.51–1.62) 0.61 (0.34–1.10) 39 1.04 (0.68–1.61) 0.89 (0.57–1.38)
Ptrendb 0.74 0.22 0.39 0.91
PTGS2-high cancer
MIR21 expression level
 Quartile 1 (lowest) 119 26 1 (reference) 1 (reference) 60 1 (reference) 1 (reference)
 Quartile 2 117 30 1.21 (0.72–2.05) 1.17 (0.69–1.98) 65 1.29 (0.90–1.83) 1.20 (0.84–1.72)
 Quartile 3 116 35 1.46 (0.88–2.43) 1.29 (0.77–2.15) 63 1.22 (0.86–1.74) 1.06 (0.74–1.51)
 Quartile 4 (highest) 118 53 2.45 (1.53–3.93) 2.28 (1.42–3.67) 82 1.86 (1.33–2.60) 1.75 (1.25–2.45)
Ptrendb < 0.0001 0.0004 0.0008 0.004
   Pinteractionc 0.007 0.0004 0.06 0.036

Abbreviations: CI, confidence interval; HR, hazard ratio.

a

The multivariable Cox regression model included sex, age, year of diagnosis, family history of colorectal cancer in parent or sibling, tumor location, disease stage, tumor differentiation, microsatellite instability, CpG island methylator phenotype, KRAS, BRAF, and PIK3CA mutations, and long interspersed nucleotide element-1 (LINE-1) methylation level. A backward elimination with a threshold of P = 0.05 was used to select variables in the final models.

b

Test for a linear trend was conducted across ordinal quartile categories (1 to 4) of tumor MIR21 expression level as a continuous variable in the Cox regression model.

c

Pinteraction values (two-sided) were calculated by the Wald test on the cross-product term of tumor MIR21 expression (ordinal quartile categories [1 to 4]) and PTGS2 expression (ordinal categories; absent [1], low [2], and high [3]) variables in the Cox regression model.