Fig. 4.

Characterization of the MagNET using microbeads. a. 1 µm diameter magnetic polystyrene microbeads were sorted from non-magnetic 1 µm polystyrene microbeads using MagNET. The enrichment of the non-magnetic beads ζ is plotted vs. flow rate. Inset: Enrichment vs. flow rate on a log–log plot for both MagNET and TEMPO. At all flow rates the enrichment of MagNET was ζ > 30× the enrichment of TEMPO. The error bars indicate standard error from replicates from flow cytometry measurement. b. As N filters are vertically stacked the enrichment at Φ = 150 ml h⁻¹ was improved ~1000×. Inset: Enrichment ζ vs. Φ on a log-linear plot, shows that enrichment ζ scales exponentially with the number of filters N. The error bars indicate standard error from replicates from flow cytometry measurement.