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. 2016 Apr 1;524(14):2845–2872. doi: 10.1002/cne.23995

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© 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Immunocharacterization of the melanopsin antibody. Top: total proteins extracted from transfected T‐Rex cells probed with anti‐1D4 (A), antimelanopsin (B), and antitubulin (C) antibodies. Bands were detected by chemiluminescence corresponding to the expected sizes of 1D4‐tagged melanopsin fusion protein monomers (54 kDa) and oligomers (over 150 kDa). Bottom: transfected T‐Rex cells expressing 1D4‐tagged melanopsin fusion proteins were immunolabeled with anti‐1D4 (D) and antimelanopsin (E) antibodies. F: DAPI staining. G: Merged image indicates colocalization. Scale bars = 20 µm.