Figure 4. In vivo distribution of the G7-Flt3L or G7-NH₂.

(A) C57BL/6 wild type mice were treated with 0.5 mg/kg Cy5.5-conjugated G7-Flt3L or G7-NH₂, i.v., once. Whole animal images at the indicated time points are shown (D1 = day 1, etc.). The white arrows indicate the location of femur bone marrow (BM). (B) Uptake ratio of Cy5.5-conjugated G7-Flt3L or G7- NH₂ in mouse BM and spleen (SP) cells. Animals were sacrificed 7 days post treatment, and the Cy5.5⁺ ratio was determined through flow cytometry analysis. (C) Uptake ratio of Cy5.5-conjugated G7-Flt3L or G7-NH2 in mouse BM and SP c-Kit⁺ or c-Kit⁻ cells. Cy5.5⁺ ratio in c-Kit⁺ or c-Kit⁻ cell populations is shown. Each experiment was repeated independently for at least three times. Average levels of at least three replicates are shown. *, P<0.05.