Table 1.
Spore germination and germ tube length of M. oryzae P131 co-cultured with cells or sterile culture filtrate of Bacillus subtilis strains SYX04 and SYX20
| Treatment | Conidia germination | Appressorial formation | Germ tubes | |||
|---|---|---|---|---|---|---|
| Number | Inhibition (%) | Number | Inhibition (%) | Length (µm) | Inhibition (%) | |
| SYX20 culture | 7 ± 0.6 d | 93.3 ± 0.6 | 5 ± 0.3 c | 93.8 ± 0.8 | 12.2 ± 2.2 c | 88.3 ± 2.1 |
| SYX20 filtrate | 27 ± 1.0 b | 72.7 ± 1.0 | 24 ± 1.0 b | 72.1 ± 0.3 | 27.6 ± 0.6 b | 73.5 ± 0.6 |
| SYX04 culture | 9 ± 1.2 d | 91.3 ± 1.2 | 6 ± 1.7 c | 93.0 ± 2.2 | 4.3 ± 0.5 d | 95.9 ± 0.5 |
| SYX04 filtrate | 23 ± 2.9 c | 76.8 ± 3.5 | 22 ± 1.2 b | 73.3 ± 1.4 | 27.8 ± 7.5 b | 73.3 ± 7.6 |
| Control | 99 ± 0.6 a | – | 86 ± 2.0 a | – | 104.3 ± 5.4 a | – |
SYX20 culture here refers to liquid culture of B. subtilis SYX20; SYX20 filtrate refers to culture filtrate of B. subtilis SYX20; SYX04 culture refers to liquid culture of B. subtilis SYX04; SYX04 filtrate refers to culture filtrate of B. subtilis SYX04; control refers to M. oryzae P131 alone. Data were first tested for normality and then analyzed using ANOVA. Significant differences between the mean values of each cohort were determined using LSD’s test (P = 0.05) following one-way ANOVA. The data is computed at P = 0.05 ± SD