Table 1.
Hepatitis E virus (HEV) detection in figatelli and pig liver sausages by RT-qPCR.
| HEV contami nation level | Sample names | EAC recovery rates (%) | Samples processed with MNV-1 |
Samples processed with mengovirus |
|||
|---|---|---|---|---|---|---|---|
| MNV-1 recovery rates (%) | HEV positive subsamples | Mengovirus recovery rates (%) | HEV positive subsamples | ||||
| Figatelli | Low | F1 | 42.29 ± 11.18 | 0.04 ± 0.02 (0/3) | 3 | 1.40 ± 0.61 (2/3) | 3 |
| F2 | 41.75 ± 7.630 | 1.34 ± 2.13 (1/3) | 3 | 5.97 ± 3.38 (3/3) | 3 | ||
| Medium | F3 | 45.94 ± 12.35 | 1.89 ± 3.08 (1/3) | 3 | 4.69 ± 2.99 (3/3) | 3 | |
| F4 | 44.83 ± 10.40 | 1.31 ± 2.01 (1/3) | 3 | 10.68 ± 6.19 (3/3) | 3 | ||
| High | F5 | 47.23 ± 11.96 | 1.16 ± 1.83 (1/3) | 3 | 4.16 ± 3.99 (2/3) | 3 | |
| F6 | 42.26 ± 9.030 | 0.15 ± 0.13 (0/3) | 3 | 4.38 ± 2.07 (3/3) | 3 | ||
| Negative control | F7 | 45.99 ± 10.89 | 7.14 ± 10.45 (3/3) | 0 | 22.11 ± 27.72 (3/3) | 0 | |
| Total subsamples with process control virus recovery rates >1% | 7 | 19 | |||||
| Pig Liver Sausages | Low | S1 | 57.27 ± 22.75 | 1.12 ± 1.46 (1/3) | 0 | 24.1 ± 25.34 (3/3) | 0 |
| S2 | 58.57 ± 25.54 | 1.97 ± 1.98 (2/3) | 1 | 16.12 ± 25.05 (2/3) | 2 | ||
| Medium | S3 | 62.35 ± 24.66 | 1.74 ± 0.30 (2/3) | 1 | 5.25 ± 8.43 (1/3) | 3 | |
| S4 | 68.81 ± 23.77 | 0.98 ± 1.38 (1/3) | 3 | 24.65 ± 32.35 (3/3) | 3 | ||
| High | S5 | 56.44 ± 26.82 | 3.75 ± 5.72 (1/3) | 3 | 15.75 ± 0.37 (3/3) | 3 | |
| S6 | 72.61 ± 22.59 | 1.43 ± 1.29 (2/3) | 3 | 15.88 ± 25.96 (2/3) | 3 | ||
| Negative control | S7 | 84.73 ± 18.20 | 14.09 ± 9.73 (3/3) | 0 | 25.50 ± 20.70 (3/3) | 0 | |
| Total subsamples with process control virus recovery rates >1% | 12 | 17 | |||||
One out of two Ct-positive HEV determinations in a single experiment set was considered a positive assay. The means of MNV-1 and mengovirus recovery rates ±SD were calculated with quantitative data obtained by RT-qPCR from undiluted RNA extracts. The number of experiments having process control recovery rates higher than 1% is indicated in brackets. The means of EAC recovery rate percentages ±SD were calculated for HEV using RT-qPCR from undiluted RNA extracts. For each sample, three experiments were performed with each process control virus, leading to six EAC recovery determinations. RNA extracts were tested twice.