Table 3.
Quantification of HEV in figatelli and pig liver sausages by RT-qPCR and RT-dPCR.
| HEV detection by RT-qPCR |
HEV detection by RT-dPCR |
HEV quantification RT-qPCR vs RT-dPCR |
||||
|---|---|---|---|---|---|---|
| Sample names | HEV-positive (out of 12) | HEV (Log10 genome copies) | HEV-positive | HEV (Log10 genome copies) | Mean (Log10 (HEV copies)RT-qPCR – Log10 (HEV copies)RT-dPCR per sub-sample) | |
| F1 | 11 | 2.90 ± 0.86 | 10 | 2.81 ± 0.41 | 0.45 | |
| F2 | 12 | 3.17 ± 0.86 | 9 | 3.12 ± 0.78 | 0.36 | |
| F3 | 11 | 4.06 ± 0.72 | 10 | 3.89 ± 0.88 | 0.16 | |
| F4 | 12 | 4.34 ± 0.68 | 11 | 4.33 ± 1.07 | 0.08 | |
| F5 | 12 | 4.97 ± 0.98 | 11 | 4.88 ± 1.08 | 0.22 | |
| F6 | 12 | 4.73 ± 0.94 | 11 | 4.58 ± 1.31 | 0.21 | |
| S1 | 0 | nd | 1 | 2.26 | / | |
| S2 | 5 | 2.08 ± 0.71 | 6 | 2.31 ± 0.12 | -0.11 | |
| S3 | 7 | 2.91 ± 0.95 | 8 | 2.77 ± 0.34 | 0.21 | |
| S4 | 11 | 3.62 ± 1.16 | 10 | 3.21 ± 0.69 | 0.56 | |
| S5 | 12 | 4.97 ± 0.25 | 12 | 4.34 ± 0.33 | 0.63 | |
| S6 | 10 | 6.32 ± 0.23 | 10 | 5.56 ± 0.11 | 0.76 | |
The results are presented as the mean of log10 (HEV genome copies) ±SD. For each sample, three experiments were performed with MNV-1 and three with mengovirus. Undiluted RNA extracts were tested twice, resulting in 12 cycle threshold (Ct) values for each sample. The number of positive Ct determinations is mentioned for HEV. HEV quantifications obtained by RT-qPCR and RT-dPCR are compared by calculating the differences for every subsamples and means of these differences are indicated for each sample.