. 2016 Aug 3;7:1096. doi: 10.3389/fpls.2016.01096

Table 1.

Protein identification by LC-MS/MS of proteins secreted by Lasiodiplodia theobromae strains CAA019 and CBS339.90.

Bands⁽¹⁾	Accession number	Proteins	Theoretical Mw (kDa)	Organism	Function⁽²⁾	Biological process⁽²⁾	Number of peptides⁽³⁾	Score MS AMANDA
1, 40	K2RRJ6	Glucose-methanol-choline oxidoreductase	72	Macrophomina phaseolina	Oxidoreductase activity, acting on the CH-OH group of donors, other acceptors	Catalysis of an oxidation-reduction reaction	2	679.27
1, 40	R1E7Q5	Putative choline dehydrogenase protein	71.3	Neofusicoccum parvum	Oxidoreductase activity, acting on the CH-OH group of donors, other acceptors	Catalysis of an oxidation-reduction reaction	2	679.27
7	R1GTC8	Putative tripeptidyl-peptidase 1 protein	64.8	Neofusicoccum parvum	Serine-type endopeptidase activity	Proteolysis	2	426.40
8	K2RUW5	Phosphoesterase	43.9	Macrophomina phaseolina	Hydrolase activity, acting on ester bonds	Hydrolase	2	663.55
9	R1GU94	Putative glucan endo–α-glucosidase agn1 protein	49.3	Neofusicoccum parvum	Hydrolase activity	Hydrolase	2	579.98
10	K2RGL3	Peptidase A1	26.9	Macrophomina phaseolina	Aspartic-type endopeptidase activity	Proteolysis	2	4710.33
6, 11	K2SBN0	β-xylanase	34.2	Macrophomina phaseolina	Hydrolase activity	Carbohydrate metabolic process, metabolic process	2	1020.33
6, 11	R1FWZ0	β-xylanase	34.8	Neofusicoccum parvum	Hydrolase activity	Carbohydrate metabolic process, metabolic process	2	1020.33
16	K2SSA3	β-galactosidase	107.8	Macrophomina phaseolina	β-galactosidase activity	Carbohydrate metabolic process	2	382.31
18	R1GH64	Putative f5 8 type c domain protein	59.2	Neofusicoccum parvum	Hydrolase activity, hydrolyzing O-glycosyl compounds	Carbohydrate metabolic process, cell adhesion	2	2398.24
19, 20, 41	K2RQR5	Peptidase A1 (Fragment)	39.9	Macrophomina phaseolina	Aspartic-type endopeptidase activity	Proteolysis	2	781.41
5, 10, 13, 15,21, 24, 30, 31, 32, 33, 37, 42, 44	R1ESA5	Putative a chain endothiapepsin	42.5	Neofusicoccum parvum	Aspartic-type endopeptídase activity	Proteolysis	2	5116.87
7, 25	K2S7L9	Glucoamylase	67.8	Macrophomina phaseolina	Glucan 1,4-α-glucosidase activity; starch binding	Polysaccharide catabolic process	2	5006.77
9, 28, 36	K2R498	Glycoside hydrolase family 71	49.2	Macrophomina phaseolina	Hydrolase activity	Hydrolase	2	2124.51
6, 12, 22, 29, 43	K2STT8	Glycoside hydrolase family 17	32	Macrophomina phaseolina	Carbohydrate metabolic processes	Hydrolase activity	2	5379.70
1, 7, 25, 26, 27, 34,40	R1GLG1	Glucoamylase	68.5	Neofusicoccum parvum	Glucan 1,4-α-glucosidase activity; starch binding	Polysaccharide catabolic process	4	9860.40

Band number corresponds to the numbering indicated in Figure 4⁽¹⁾. The GO terms correspondent to the function and the biological processes⁽²⁾ of the proteins were searched with Interpro (Hunter et al., 2012). The subcellular localization was deduced with Bacello (Pierleoni et al., 2006): all proteins were inferred to be extracellular. Only proteins with two unique peptides identified were included in the table. The number of peptides⁽³⁾ is the number of peptides identified per band.