Table 4.
Colonization of tomato roots by Trichoderma harzianum wild-type T34 and the amdS transformants.
| Trichoderma actin | Tomato actin | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Condition | Ct | SD | Qty* | SD | Ct | SD | Qty** | SD | Ratio*** |
| T34 | 20.77 | 1.33 | 2.04 | 0.42 | 29.15 | 0.85 | 0.88 | 0.31 | 2.45a |
| amdS6 | 20.30 | 0.57 | 2.19 | 0.18 | 28.67 | 0.66 | 1.06 | 0.24 | 2.12a |
| amdS122 | 20.17 | 1.07 | 2.23 | 0.34 | 28.80 | 1.40 | 1.01 | 0.52 | 2.71a |
Fungal DNA present on the tomato roots 20 h after inoculation was quantified by real-time PCR.
*
Quantity of fungal DNA (ng) referred to Trichoderma actin gene.
**
Quantity of plant DNA (ng) referred to tomato actin gene.
***
Proportion of fungal DNA vs. plant DNA. Values are means of three biological replicates with the corresponding standard deviation. Values followed by the same letter are not significantly different (P < 0.05).