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Fluorescent in situ hybridization of Rhopr-CRZR transcript in the brain of 5th instar R. prolixus. (A) Confocal microscopy image of the brain revealing a group of seven bilaterally paired medial cells on the dorsal surface. (B) Diagram illustrating the location of cells in the brain containing the Rhopr-CRZR transcript. The fluorescent in situ hybridization was performed three times with eight CNSs each time. SOG, suboesophageal ganglion.
