Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 3;10:357. doi: 10.3389/fnins.2016.00357

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Hamoudi, Lange and Orchard.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

The effect of Rhopr-CRZR on ecdysis in R. prolixus. (A) 4th instar R. prolixus were injected with 2 μg of dsARG or dsCRZR 10 days post feeding. Ecdysis took place 11–16 days post-feeding. There was no significant effect on the timing of ecdysis between dsARG RNA and dsCRZR injected insects; however, dsCRZR injected insects were observed to undergo ecdysis a day earlier causing a shift in the median (vertical line). (B) Temporal expression analysis of Rhopr-CRZR transcript in the CNS by qPCR indicated that the receptor expression is not up-regulated around the timing of ecdysis. The expression was analyzed in 4th instar CNS 3 days before ecdysis (DBE), 2 DBE, 1 DBE, 2 h post-ecdysis (HPE) into 5th instar, 4 HPE, 1 day post-ecdysis (DPE), 3 DPE, and 6 DPE. Fold difference is relative to transcript levels of 2 HPE. The experiment was repeated on three biological replicates with three technical replicates each. Values represent mean ± error of mean.