FIGURE 5.

Effect of iron deficiency on the level of peroxidation of the chloroplast lipids of P. tricornutum. High temperature thermolumine-scence glow curves (HTL2 band) of P. tricornutum cells from both Fe-replete and Fe-deficient cultures. Cell suspensions from 21 days cultures (equivalents to 7.5 μg Chl) were adsorbed by filtration on a piece of filter paper that was pressed against the copper film, dark-incubated for 10 min at 20°C, and cooled to 10°C for 1 min. Luminescence emission was then recorded while warming samples from 10 to 160°C at a heating rate of 0.1°C s^-1. For further details see “Material and Methods” section.