Table 1.

Physiological and biochemical parameters of Phaeodactylum tricornutum cells from Fe-replete and Fe-deficient cultures.

Parameter	Fe-replete	Fe-deficient
Growth rate (μ, days-1)	0.115 ± 0.014	0.081 ± 0.012
Chl a (mg Chl. g-1 wwt biomass)	5.5 ± 0.9	3.1 ± 0.7
Chl c (mg Chl. g-1 wwt biomass)	1.2 ± 0.5	1.3 ± 0.3
O2 evolution (μmol O2. mg-1 Chl. h-1)	115 ± 12	51 ± 7
O2 consumption (μmol O2. mg-1 Chl. h-1)	59 ± 4	11 ± 4
Fv/Fm	0.615 ± 0.018	0.404 ± 0.023

Specific growth rates were calculated as μ = (ln OD₂ – ln OD₁)/Δt, where OD₁ and OD₂ are optical density at 750 nm of cells after 7 and 21 days of culture, respectively, and Δt the corresponding time interval. Chlorophyll concentration is expressed as mg of Chl per g of wet weight biomass (wwt). Data represent the mean ± SD of three replicate determinations from separate cultures. Measurements were done using cells harvested after 21 days of culture.