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. 2016 Aug 3;7:1211. doi: 10.3389/fmicb.2016.01211

FIGURE 3.

FIGURE 3

Expression levels of HIV-2 GL-AN Vpx and its d7P mutant. Expression levels of wild-type (WT) and mutant Vpx proteins in transfected 293T cells were examined by Western blotting analysis. Results of two independent experiments are shown. Relative protein amounts used for blotting are indicated in parentheses. As WT samples, an eighth and a sixteenth amounts were also used for comparative quantification purpose. (-), pEF1/Myc-HisA (empty vector); WT, pEF-Fvpx; d7P, pEF-Fvpx/d7P mutant (Miyake et al., 2014a,b).