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Expression of PPM-deleted Vpx/Vpr mutant proteins from various HIV/SIVs. Expression of PPM-deleted Vpx/Vpr proteins (A–C) in transfected 293T cells as monitored by Western blotting analysis is shown. Viral groups (Figure 1A) are indicated. Each deletion mutant (d2P, d4P, d5P, and d7P) lacks the entire PPM sequence (Figure 2). Amounts of sample cell lysates, antibody concentrations, and exposure time were adjusted individually for appropriate band intensity as shown.
