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. 2016 Aug 3;10:55. doi: 10.3389/fncir.2016.00055

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Copyright © 2016 Ballout, Frappé, Péron, Jaber, Zibara and Gaillard.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Development and maturation of axons of grafted GFP neurons from day 2 (D2) to day 30 (D30) post-transplantation. (A–C, G–I) Immunohistochemistry for GFP (green) and DCX (red) shows that many GFP+ fibers co-express DCX. (D–F, J–O) Immunohistochemistry for GFP (green) and PSA-NCAM (red) shows GFP axons co-expressing PSA-NCAM. GFP+ fibers co-expressing DCX or PSA-NCAM leave the graft and extend through the cortex and the corpus callosum ipsilateral to the transplant. Note that co-expression of DCX or PSA-NCAM by axons of grafted neurons decreased in relation with the post-transplantation time, indicative of the maturation of GFP axons. cc, corpus callosum; Cpu, caudate putamen; Cx, cortex; Scale bars: A–C, 55 μm; D–O, 80 μm.