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Codon usage bias in the E. coli pangenome estimated by four different measures, viz., (A) CDC, (B) CAI, (C) Nc, and (D) Nc′. Correlation between CAI and gene expression level was examined in (E) strain-specific genes, (F) lowly-shared genes, (G) moderately-shared genes, (H) highly-shared genes, and (I) core genes, respectively.
