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AtSIZ1 is ubiquitinated by COP1 in vitro. Ubiquitination reactions were performed in the presence or absence of rabbit E1, UbcH5b (E2), MBP-COP1 (E3), His6-Ubiquitin, and His6-AtSIZ1-HA. After reactions were complete, ubiquitinated AtSIZ1 (A) and COP1 (B) were detected by Western blot analysis using anti-HA and anti-MBP antibodies, respectively.
