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. 2016 Jul 20;2(7):e00131. doi: 10.1016/j.heliyon.2016.e00131

Fig. 9.

Fig. 9

Establishment of HeLa-S3 cell lines with tetracycline-inducible INCENP. Isolation of HeLa-S3 clones inducibly expressing 6Myc-INCENP-WT, -S894A, or -S894D. After treatment of each clone with doxycycline (Dox), cell lysates were immunoblotted with the indicated antibodies. Clones #13, #8, and #13 were used in this study. See Fig. S4 for uncropped western blot images in Fig. 9. Black boxes indicate the cropped regions. Molecular sizes (kDa) are based on prestained protein markers.