Table 2.

IC50 of Bz and 1g on epimastigotes (Epi), trypomastigotes (Tryp) amastigotes (Ama), Vero cells and Cardiomyocytes (Vero/CM) incubated in medium with and without hemin. Results represent the mean and standard deviation of at least three experiments in duplicate.

Medium	1g				Bz
	Epia	Trypb	Amac	Vero/CMd	Epia	Trypb	Amac	Vero/CMd
Without heme	19.5 ± 2.5	>100	12.2 ± 2.6	>100	2.9 ± 0.6	13 ± 5.7	3.25 ± 0.3	>100
With heme*	<1**	11.7 ± 4**	6.3 ± 2.5**	>100	9.5 ± 5.2**	12 ± 6.5	2.5 ± 1.4	>100

*30 μM of heme diluted in BHI, RPMI and DMEM medium.

**Statistically significant p < 0.05 using one-way ANOVA.

^aIC₅₀ (Concentration that inhibits the growth of 50% of T. cruzi Y epimastigote forms within 72 h). Viability was measure counting motile parasites in Neubauer chamber.

^bIC₅₀ (Concentration that kills 50% of T. cruzi Y trypomastigotes within 24 h). Viability was measure using resazurin.

^cIC₅₀ (Concentration that inhibits 50% of T. cruzi Y amastigotes after 72 h of treatment). Measured by counting the number of parasites stained with GIEMSA.

^dCC₅₀ (Concentration that inhibits 50% of host cell growth after 72 h of treatment). Viability was measure using resazurin.