Figure 2.

Localization of ZO-1 and GM130 is altered by H. pylori CagA. (a-b) Cells were infected with the CagA positive strain EPIYA ABCCC and were stained with anti-ZO-1 or anti-E-cadherin antibodies (green) and DAPI (blue). Arrows in panel (a) indicate the loss of signal of E-cadherin and in panel (b) show delocalization of ZO-1. (c) MCF-10A cells were infected with H. pylori positive strains ABCCC and ABD, stimulated with IL-1β, or both infected and stimulated during acini morphogenesis. The acini structures were stained with anti-GM130 antibody (green) and DAPI (blue) and confocal microscopy sections were made at 50% depth of the acini. The bottom panels show an enhanced fragment of the acini to see in more detail the GM130 distribution. Figures are representative of three independent experiments performed in duplicate or triplicate.