Figure 4.

Cell wall integrity assays of C. higginsianum to cell wall inhibitor. (A) CH-1, ▵ChMK1-1, and ChMK1-Com treated with indicated cell wall inhibitor in PDA. Images were taken after 7 days of incubation on PDA with 0.01% SDS, 300 μg/ml Calcofluor White (CFW), and 300 μg/ml Congo Red (CR). (B) Inhibition of the radiated growth of CH-1, ▵ChMK1-1, and ChMK1-Com on the PDA with cell wall inhibitor. Means and standard errors were calculated from three replicates. Asterisks indicate statistical differences from the ▵ChMK1-1 (P < 0.05).