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. 2016 Aug 3;7:108. doi: 10.3389/fendo.2016.00108

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Copyright © 2016 Jacobs, Sadie-Van Gijsen, van de Vyver and Ferris.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of vanadate on the expression of late markers of adipogenesis. Cells were treated with adipogenic media (AM) or AM plus 10 μM vanadate (AMV) for the number of days as indicated, and the expression of adipocyte protein-2/fatty acid binding protein-4 (aP2/FABP4), adipsin, and fatty acid synthase (Fasn) was measured with qRT-PCR. All target gene measurements were normalized to the housekeeping gene acidic ribosomal phosphoprotein (ARBP). The expression of aP2/FABP4, adipsin, and Fasn in AM-treated cells was set as 1. The graph presents the combined data of n = 3 for bmMSCs and n = 4 for pfMSCs. Statistically significant differences are indicated as **P < 0.01 or ***P < 0.001.