Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 3;7:330. doi: 10.3389/fphys.2016.00330

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Ley, Romantsik, Vallius, Sveinsdóttir, Sveinsdóttir, Agyemang, Baumgarten, Mörgelin, Lutay, Bruschettini, Holmqvist and Gram.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Hemorrhage distribution following preterm rabbit pup IVH. Following IVH in preterm rabbit pups, the distribution of the hemorrhage was investigated. Rabbit pups with IVH or sham controls were euthanized at 72 h of age followed by saline and freshly prepared 4% PFA perfusion. Afterwards the brains were dissected out from the skulls and immersed in 4% PFA. Brains were prepared and sections from Control and IVH animals, at the levels of rostral forebrain (Level 1), caudal forebrain (Level 2), rostral midbrain (Level 3) and caudal midbrain (Level 4), were stained for hematoxylin and eosin (HE) and peroxidase activity of Hb (Peroxidase) as described in the Materials and Methods Section. Microscope analyses were performed on a wide-field Olympus microscope (IX73) and slide scanning were performed on a Hamamatsu NanoZoomer 2.0-HT Digital slide scanner: C10730. Scanning was performed with a 40 × magnification lens. Scale bar indicate 2.5 mm and is applicable for all images.