Figure 2. The presence of RGD leads to growth at a similar rate as collagen, which are both twice as fast as pure alginate.

(a) Growth of M6 cells with RGD-alginate, collagen/alginate composit, and pure alginate. (b) Growth of M6 cells within the 5.29, 2.85, and1.85 kPa RGD-alginate gel. Mean is shown from four separate experiments, with 40 to 100 MCAs at each time point for each culture condition. Error bars are standard error of the mean. For statistical analysis we did t-test or Mann-Whitney test depending on the data distribution for each time point and P-values are between 0.3–0.6 which shows there is not a statistically significant difference in the results. (c) The slope of the single linear fit of the Gompertz equation can be interpreted as MCA growth rate.