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. 2016 Aug 3;6:31085. doi: 10.1038/srep31085

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Copyright © 2016, The Author(s)

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(A) Relative expression of p18 mRNA in MEFs transfected with empty vector or vector expressing p18 cDNA. The results are presented as the mean ± SD of three independent experiments; **P < 0.01. (B) Western blot analysis of whole cell extracts from MEFs transfected as in (A). Actin serves as a loading control. (C) Histograms of DNA contents in cells transfected as in (A). (D) p18 overexpression significantly increases the proportion of cells in G1 phase. Data are shown as mean ± SD; n = 3, *P < 0.05. (E) Alkaline phosphatase (AP) staining of iPSC colonies generated from control cells or cells overexpressing p18. (F) p18 overexpression blocks reprogramming. AP-positive colonies were counted and normalized to cells transfected with empty vector. Data are shown as mean ± SD; n = 3, **P < 0.01.