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. 2016 Aug 3;6:30949. doi: 10.1038/srep30949

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Copyright © 2016, The Author(s)

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(A) NTA induced the β-galactosidase activity of the PCZ reporter strain. Overnight PCZ culture was freshly inoculated with a 0.1% dilution in LB medium (blue square) or LB medium supplemented with 7.5 mM NTA (red circle); samples were collected at the specified time points for β-galactosidase activity assay. (B) Spermidine (Spd) stimulation of TTSS was independent of calcium depletion. Overnight cell culture of strain PCZ was inoculated at 1:1000 ratio in the CaCl₂-containing mininal medium (MM) (blue square) or in the CaCl₂-containing MM medium supplemented with Spd (0.7 mM) (green diamond).