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. 2016 Aug 3;6:30892. doi: 10.1038/srep30892

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Copyright © 2016, The Author(s)

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(A) Fixed CHO-K1 cells expressing paxillin-EGFP were photo-bleached with 100–200 scans of the CLSM in 100 × 100 pixel regions of interest (ROIs). The zoomed in ROI shows how the fluorescent intensity bleaches over time. Frame numbers are shown in the upper right corner. (B) Intensity decay in an ROI shown as a function of the number of images collected. Scale bar is 10 μm for the whole cell image and 2 μm for the ROI images.