Table 1. Summary of Line Scanning Settings.

Configuration	Software Scan Speed Setting	Number of Lines Scanned	Pixel Dwell Time	Total Pixel Dwell Time (Pixel Dwell Time x Number of Lines Averaged)
1	5	1	12.61 μs	12.61* 1 = 12.61 μs
2	6	2	6.30 μs	6.30* 2 = 12.60 μs
3	7	4	3.15 μs	3.15* 4 = 12.60 μs
4	9	8	1.58 μs	1.58* 8 = 12.64 μs
5	12	16	0.79 μs	0.79* 16 = 12.64 μs

Line scan settings using the standard CLSM and the 488 nm laser at 5% power. The total image acquisition time was 1.51 s while the number of line scans and the pixel dwell time were varied to mimic laser pulsing. The “pulse width” was essentially the pixel dwell time and the number of pulses was the number of line scans. The time between pulses was on the milliseconds time scale and was related to the time for the laser to scan back to the start of the line between line scans.