Table 2. Laser percentage to power conversions.

Laser Line (nm)	Power (%)	Average Power (μW)	Experiments that used this power	Figures where this power was used
473	40	500	ns pulsing	Figure 3
473	5.6	67	ns pulsing	Figure 3
488	1	11	Protrusion-retraction Assay	Figure 6
	2	22	Imaging	Figures 4 and 7
	5	50	Variable line scanning, EGFP-Blue Excitation	Figure 4, Supplemental Figure 4
	5.6	57	OxyFlourTM	Supplemental Figure 1
	20	260	TMRM and morphology of the mitochondria assays	Figures 5 and 7
543	1	4	TMRM Imaging	Figure 5
	25	97	mCherry Photobleaching	Supplemental Figure 2
488	10	184	Wide-field/Spinning disk	Supplemental Figure 3

Laser powers were measured using a Coherent FieldMaxII-TO laser power meter. Note that powers were measured with the 10x/0.3 NA lens and were not necessarily measured every time an experiment was conducted.