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. 2016 Aug 3;6:30698. doi: 10.1038/srep30698

Figure 5. Effects of rSLURP-2 on the growth of Het-1A cells.

Figure 5

(A) Influence of rSLURP-2, rSLURP-1, and ws-Lynx1 on Het-1A cell growth (% of control, n = 6–12, mean ± S.E.). The Hill equation (y = A1(100%-A1)/(1+([protein]/IC50)nH)) was fitted to the data measured using WST-1 reagent. The data for rSLURP-1 were obtained from ref. 24. The calculated EC50, nH and A1 parameters were 7.6 ± 1.0 nM, 1.5 ± 0.4 and 116 ± 1% (rSLURP-2/Het-1A), and 4.3 ± 0.6 nM, 1.4 ± 0.2 and 60 ± 1% (rSLURP-1/Het-1A), respectively. (B) Effects of rSLURP-2 (1 μM), Atr (1 μM), Mec (10 μM), α-Bgtx (1 μM), MII (1 μM) and their co-application on the growth of Het-1A cells after 48 hours. Each bar is the mean ± S.E. of four independent experiments performed in triplicate. The pairwise statistical analysis of the data groups measured with and without rSLURP-2 was done using t-test. Data indicated as **(p < 0.01) and ***(p < 0.001) are significantly different from each other. Multiple comparisons of all the data groups with the ‘control’ group and with the ‘rSLURP-2’ group were done using ANOVA followed by special Dunnett´s multiple comparisons test. Data marked with ¤ and #(p < 0.05), ¤¤ and ##(p < 0.01), ¤¤¤ and ###(p < 0.001) are significantly different from ‘rSLURP-2’ and the ‘control’, respectively. (C) Effects of rSLURP-2 (1 μM) and its co-application with atropine (Atr, 1 μM), Mec (10 μM), α-Bgtx (1 μM), or α-conotoxin MII (MII, 1 μM) on the morphology of Het-1A cell nuclei after 24 and 48 hours. The cells nuclei were colored with Hoechst 33342 and propidium iodide.