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. 2016 Aug 3;6:30938. doi: 10.1038/srep30938

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Samples of mouse serum were assayed using IgG3- and IgM-specific ELISAs. Samples from 11 different mice at age 25–29 weeks were analysed, among which 5 animals were immunized with RBCs. Arrows indicate serum sample of mouse no. 5, whose splenocytes were used in fusion resulting in M18 antibody generation. Agglutination was assessed for samples diluted 100 times. Error bars correspond to SD of concentration measurements.