Table 2. Analysis of specific antibody sequences.
| Fusion | Genes coding for a variable fragment of the specific mAba (identity with the closest V-region) |
Mutationsb HC/LC | Amino acid substitutionsc HC/LC | Isotype | |
|---|---|---|---|---|---|
| Heavy chain (HC) | Light chain (LC) | ||||
| M | IGHV1-9*01 F (94%)IGHJ2*01 F (94%)IGHD2-4*01 F | IGKV1-99*01 F (95%)IGKJ4*01 F (100%) | 16/6 | 12/3 | IgG3 κ |
| O | IGHV6-7*02 F (96%)IGHJ4*01 F (96%)IGHD1-1*02 F | IGKV1-110*01 F (96%)IGKJ1*01 F (100%) | 5/2 | 3/2 | IgM κ |
| Q, U | IGHV4-1*02 F (97%)IGHJ3*01 F (92%)IGHD5-8*01 | ndd | 3/nd | 0/nd | IgM λ |
aSequence analysis revealed that all specific clones derived from a single mouse produce exactly the same mAb.
bMutations with respect to the germline sequence were analysed within FR1-FR3.
cAmino acid substitutions with respect to the germline sequence were analysed within FR1-FR3.
dNot determined. Despite many efforts we did not manage to design λ-specific primers allowing for selective amplification of a productive assemblage of λ VJ-genes.