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. 2016 Aug 3;6:30896. doi: 10.1038/srep30896

Figure 2. Subcellular distribution of Rbfox2 is altered in HLHS right ventricles.

Figure 2

(a) Immunofluorescence (IF) of Rbfox2 in right ventricle sagittal sections of infants with HLHS or Tetralogy of Fallot (control). Cardiomyocytes were marked with cardiac troponin I. Nuclei were stained with DAPI/TO-PRO-3. Fluorescence images were obtained at 80X magnifications with a confocal laser-scanning microscope (LSM 510META, Carl Zeiss) at the University of Texas Medical Branch imaging core facility. (b) Representative images of GFP (control), GFP-tagged wild type (Rbfox2WT) and nonsense mutant (Rbfox2Nonsense) of Rbfox2 protein in COS M6 cells. (c) WB analysis of GFP-Rbfox2WT and GFP-Rbfox2Nonsense protein in COS M6 cells using anti-Rbfox2 antibody. GAPDH WB was used as a loading control. (d) Percent inclusion of Ank2 exon 41 in control (vector), GFP-Rbfox2WT or Rbfox2Nonsense mutant expressing COS M6 cells determined by qRT-PCR (n ≥ 3). Each reaction was done as triplicates for 3 different experiments and significance was calculated using one-way ANOVA.