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. 2016 Aug 3;6:30792. doi: 10.1038/srep30792

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Copyright © 2016, The Author(s)

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(A) Neurons at d35 of differentiation stained positive for MAP2 and βIII-tubulin. Scale bars indicate 100 μm. (B) SNaPshot analysis of the allelic expression ratio of the paternal (G) and the maternal (A) allele of UBE3A. The G/A ratio decreased at day 28 and day 35 in the time course of neuronal differentiation, indicating that silencing of the paternal allele is a late event.