Figure 3. Reduced phosphorylation of STAT3, ERK and p65/NF-κB in hyperthyroid livers.

(A) % survival of euthyroid and hyperthyroid male mice after treatment with 5 and 20 mg/kg LPS. (B) Western blots of the indicated proteins performed with liver extracts from control euthyroid (Euth.) and hyperthyroid (Hyperth.) mice. Mice were treated with vehicle or 5 mg/kg LPS for 5 h. Each band represents pooled extracts of 4–6 animals. (C) Similar experiment in euthyroid and hyperthyroid mice treated with 20 mg/kg LPS for 45 min and 5 h. Extracts from 4–6 animals were pooled except in the hyperthyroid group treated with LPS for 5 h, where only 2 mice survived. Full-length blots are presented in Supplementary Figure 9.