Figure 2. Mbnl3^ΔE2 muscles do not show altered Insr exon 10a splicing.

(a) Insr exon 10a inclusion is not significantly different in male Mbnl3^ΔE2 soleus muscles when compared to male Mbnl3^+/+ soleus muscles at 11 months of age. Data are standard error of mean (n = 3 soleus muscles from independent mice for each genotype). Exon numbers are annotated based on Refseq from UCSC genome browser (NCBI37/mm9). (b) Relative Insr exon 10a inclusion was quantitated by qPCR analysis using exon-exon boundary spanning primers specific to exon 10a (exon 10a–11) and to a constitutive exon (exon 9–10) at 11 months of age. Expression levels of Insr exon 10a were normalized to that of Insr exon 9. Data are standard error of mean (n = 3 soleus muscles from independent mice for each genotype; each sample was repeated in triplicate).