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Comparative analysis of HT-29 cells colonization by E9034A, isogenic mutants (E9034AΔlngA, E9034AΔlngB, E9034AΔlngC, E9034AΔlngD, E9034AΔlngH, and E9034AΔlngP) and complemented strains. Giemsa staining of adherence assays on HT-29 cells showing differences in the number of bacteria attached to the cells. The complementation of the mutants by the addition of a wild-type copy of the mutant gene in trans restored the wild-type phenotype. Photographs taken at 100x.
