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. 2016 Aug 3;7:1201. doi: 10.3389/fmicb.2016.01201

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Copyright © 2016 Saldaña-Ahuactzi, Rodea, Cruz-Córdova, Rodríguez-Ramírez, Espinosa-Mazariego, González-Montalvo, Ochoa, González-Pedrajo, Eslava-Campos, López-Villegas, Hernández-Castro, Arellano-Galindo, Patiño-López and Xicohtencatl-Cortes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LngP is the prepilin peptidase of LngA. (A) Production of the LngA subunit detected by Western-blot of bacterial lysates, using a specific anti-CS21 serum. Anti-CS21 serum revealed a protein with a Mr (relative molecular mass) of 22-kDa in the E9034A strain, and one of 25-kDa in the lngP mutant, consistent with LngA (pilin) and pLngA (prepilin), respectively. (B) Phenotypic characterization by immunofluorescence assay of the E9034A, E9034AΔlngA, E9034AΔlngP, and E9034AΔlngP (pUClngP) strains. The DNA was stained with propidium iodide (red) and CS21 structures were visualized with anti-IgG antibodies conjugated with Alexa fluor 488 (green). Photographs taken at 63x.