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LngB protein is a minor pilin subunit. (A,B) Immuno-gold assay, using specific anti-LngB serum, showing that LngB is probably localized at the tip of CS21 in the E9034A strain. (C) As negative control the E9034AΔlngB strain was used, absence of gold labeling was observed. (D) Measurements of CS21 length by confocal microscopy of the E9034A, E9034AΔlngB, and E9034AΔlngB (pUClngB) strains were plotted. This data was obtained from 20 different fields of two different experiments.
