Table 2.

List of primers used in this study.

Primer	Sequence	Use
LngA-FC	5′ TCGCCATGGGGATCCAATTACGTAAACAACGT-3′	Cloning of lngA in pUC18
LngA-RC	5′ TTGAAGCTTTTAACGGCTACCTAAAGTAATTG-3′	Cloning of lngA in pUC18
LngA-FP	5′ TGCGGATCCGTGATCTGAAGAAAAATAA-3′	Cloning of lngA in pJET1.2/blunt
LngA-RP	5' TGTGAGAAGGTACTAGCCTATCATATT-3′	Cloning of lngA in pJET1.2/blunt
LngB-FM	5′ CACAGAGACAATTTTATGAAAATGAGAGGCTTCGTGTAGGCTGGAGCTGCTTC-3′	Mutagenesis
LngB-RM	5′ CATTTTATCCTCTCCATTACATTAGGTTTGTGGTTCTGTACTATATGAATATCCTCCTTAG-3′	Mutagenesis
LngB-FS	5' GTAGAGCTCAGGTAAACTCAATTACTTTAGGTAGCCGT-3′	Screening mutants
LngB-RS	5′ TGCAAGCTTCAGGAGATGCCAGTGCACTCG-3′	Screening mutants
FlngBALL	5′ AGAAGGAGATATAACTATGAAAATGAGAGGCTTCACACTTCTGGAGATGATTATCACTCTCGC-3′	Cloning of lngB in pLATE31
RlngBALL	5′ GTGGTGGTGATGGTGATGGCCGGTTTGTGGTTCTGTACTGCACCAGGTTGTGATA-3′	Cloning of lngB in pLATE31
LngC-FM	5′ AATGGAGAGGATAAAATGAGAGCAAAATGGGTGTAGGCTGGAGCTGCTTC-3′	Mutagenesis
LngC-RM	5′ACTTCATATTCTTATACCTGACATTCATTGAGAGTCGATAACCAGTACATATGAATATCCTCCTTAG-3′	Mutagenesis
LngC-FS	5′ CAACCATGGGGATCCCAGAACCACAAACCTAATG-3′	Screening mutants
LngC-RS	5′ TGATGAGAATTCCTGCAATGCCAATAAAACTAACATC-3′	Screening mutants
FlngCALL	5′ AGAAGGAGATATAACTATGAGAGCAAAATGGGGTGTTTTTTTCTTCCTTAGTATTCTTTCTTCGAG-3′	Cloning of lngC in pLATE31
RlngCALL	5′ GTGGTGGTGATGGTGATGGCCTTGAGAGTCGATAACCAGTACGTTGTTTC-3′	Cloning of lngC in pLATE31
LngD-FM	5′ ACTGGTTATCGACTCTCAATGAATGTCAGGTATAAGAATGTGTAGGCTGGAGCTGCTTC-3′	Mutagenesis
LngD-RM	5′ CGATAATAGTCAATACCTACAACCATTGCGATGGTATTATCAATATGAATATCCTCCTTAG-3′	Mutagenesis
LngD-FS	5′ GTCCCATGGGGATCCGTTTTCTTCAGAACAATAT-3′	Screening mutants
LngD-RS	5′ CCATAAGAGCTCCAGCGCAATTTTTTCATC-3′	Screening mutants
LngH-FM	5′ CTTGTACATAAAGTTAAAATGACGGGCCTTGCAGTCGTTGTGTAGGCTGGAGCTGCTTC-3′	Mutagenesis
LngH-RM	5′ CAAAACTGATAGAGATAAATACGTTGTTTTTTATTGAATTTTTTCAAGTAATACCTCATATGAATATCCTCCTTAG-3′	Mutagenesis
LngH-FS	5′ AGAGAATTCCCGGGAAAGTACAGGCTG-3′	Screening mutants
LngH-RS	5′ GAGTCATAGATCGGTAATCCTGAAAGCTTCAT-3′	Screening mutants
LngP-FM	5′ ATGTATGTTGAAATCGGCGTTTTCTTTTTTTTATTCATTACAGTGTAGGCTGGAGCTGCTTC-3′	Mutagenesis
LngP-RM	5′ ATCTCTATGCATTTCTCATAGAAATAAGAGAAAGTATTCATAAACATATGAATATCCTCCTTAG-3′	Mutagenesis
LngP-FS	5′ GTTGGATCCAGATTTAGTGGGCCT-3′	Screening mutants
LngP-RS	5′ GATGTCGACGAGCTCTATAGATATTAAATCTCTAT-3′	Screening mutants