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PCR-RFLP analysis of HLA-G* 0105N null allele in subjects with RM. The PCR products were digested overnight with PpuM-1 restriction enzyme. The lane MW represents the 100 bp DNA ladder. Lanes 1 – 7 show products after restriction digestion with normal pattern (108 and 168 bp). Lane 8 shows the product after digestion with heterozygous pattern (108, 168 and 276 bp).
