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. 2016 Aug 3;6:31037. doi: 10.1038/srep31037

Figure 3. In vitro conformation evaluation and cell encapsulation studies and in vivo subcutaneous implantation for the SF hydrogels.

Figure 3

(a) SF hydrogel transmission electron micrographs after incubation in PBS at 37 °C for 1 (left) and 10 days (right). The black arrows indicate nanofibrils and the red arrows indicate nanofibrils aggregates (Scale bar:200 nm). (b) Macroscopic images of explants retrieved after 2 weeks of subcutaneous implantation (scale bar: 5 mm). (c) SF hydrogel ATR-FTIR spectra after 2 weeks of implantation. SF solution: 16 wt. %; Silk/HRP: 0.26‰ (wt/wt). (d) Cell viability after encapsulation analyzed by MTS assay (n = 9). (e) Live/dead staining for the ATDC-5 cells whithin the SF hydrogels from 1 to 10 days (scale bar: 100 μm). (f) H&E staining of the explants (scale bar: 400 μm). **P < 0.01, ***P < 0.001, ****P < 0.0001.