Fig. 3.

Fragment AAV vector design and transduction. (a) A single plasmid with an intact transgenic cassette is depicted. (b) During AAV vector production different sized genome fragments of the over-sized transgenic cassette are packaged. Fragments of both polarities are packaged (gray and black lines) starting at the 3′ end. Opposite polarity fragments containing regions of complementarity can anneal followed by strand synthesis to generate a large transgenic cassette. Contaminating fragment genome species below half of the intended transgenic cassette do not contain strand complementarity and are non-productive for transduction. Black box AAV inverted terminal repeat