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. Author manuscript; available in PMC: 2016 Aug 3.
Published in final edited form as: Gene Ther. 2008 Aug 14;16(1):43–51. doi: 10.1038/gt.2008.134

Figure 2.

Figure 2

Chr19 promoter function in human embryonic kidney cells. 293 cells were transfected with plasmid constructs containing different promoter elements from the Chr19 region depicted in Figure 1. (a) The full-length (347 bp) Chr19 element was investigated for promoter activity, paired with gfp, in the forward (F) and reverse (R) orientations. Fluorescent microscopy was performed 24 h after the transfection of 5 μg of plasmid DNA in a 10 cm plate. (b) Promoter activity of the Chr19 region was further dissected using the α-1 antitrypsin (AAT) gene product as the reporter. The primers used for Chr19 region amplification are depicted in Figure 1. The AAT concentration was determined in culture supernatant by ELISA 24 h post-transfection of 5 μg plasmid DNA in a 24-well plate. The chicken β-actin promoter (CBA) was used as a relative control. The results were averaged from four independent experiments and standard deviation is shown. **P<0.01 vs baseline without promoter. ELISA, enzyme-linked immunosorbent assay.