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. Author manuscript; available in PMC: 2016 Aug 3.
Published in final edited form as: Gene Ther. 2008 Aug 14;16(1):43–51. doi: 10.1038/gt.2008.134

Figure 3.

Figure 3

Chr19 enhancer function in human embryonic kidney cells. (a) 293 cells were transfected with 0.5 μg of plasmid constructs containing different elements immediately upstream of the mini-CMV promoter. The primers used for Chr19 region amplification are depicted in Figure 1. The α-1 antitrypsin (AAT) gene was used as the reporter and AAT concentration was determined in culture supernatant by ELISA 24 h post-transfection. (b) The full-length Chr19 element–mini-CMV pairing was also investigated for AAT production when located between AAV's inverted terminal repeats (TRs) in a plasmid context. The result was the average of five separate experiments and standard deviation is shown. **P<0.01 or *P<0.05 vs the mini-CMV promoter. AAV, adeno-associated virus; ELISA, enzyme-linked immunosorbent assay.