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. 2016 Aug 3;7:1142. doi: 10.3389/fpls.2016.01142

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Copyright © 2016 Kettles and Kaloshian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Me47 can utilize selected isothiocyanates as substrates. Glutathione depletion assay analysis of substrates (A) benzyl isothiocyanate (BITC), (B) phenylethyl isothiocyanate (PEITC), and (C) allyl isothiocyanate (AITC), incubated with buffer control, commercial equine GST (eqGST; Sigma), recombinant N-terminal His-tagged Me47 (Me47) and non-enzymatic recombinant N-terminal His-tagged GroEL (GroEL).