Fig. 1.

The total EGFR protein expression, phosphorylation of EGFR and EGFR peptides phosphorylation in primary AML samples. a Total EGFR protein expression was measured using RPPA of 511 adult AML samples. Total EGFR protein was highly expressed in 11 % (57/511) of the adult AML samples versus the CD34+ normal bone marrow (NBM) samples. The distribution density of total EGFR expression was depicted in the figure. b Similarly, the EGFR protein phosphorylation levels were measured using RPPA of the same 511 adult AML samples. EGFR protein phosphorylation levels were 18 % (93/511) higher in adult AML patient samples compared to the CD34+ NBM samples. c The EGFR peptide phosphorylations were measured using PepChipTM Kinomics microarray system of 179 AML patients. The median of four EGFR peptides with different phosphorylation sites was used to make the graph